DISCUSSION . A. ENDOSPORE STAIN . Alternate Endospore Stain. Endospore stained slide with + control (Bacillus) on left, negative control on right and unknown in center. Growth On DTC agar. Malachite green staining step of endospore stain procedure. It is a simpler but less robust procedure. The organism was able to grow in both aerobic and anaerobic tubes. DISCUSSION. Endospore stains require heat to drive the stain into the cells. For the staining of endospores, a first article was published by Dorner in the year 1922. For best results, follow this procedure … For a endospore stain to be successful, the temperature of the stain must be near boiling and the stain cannot dry out. Name two endospore-producing genera of bacteria. Endospore staining is a type of special staining method that makes the use of differential staining procedure to highlight the presence or absence of endospores. THE VIRTUAL EDGE: Lab 4 Bacterial Staining Techniques III Endospore Stain Results: Lab 4 / Endospore Stain / Capsule Stain / Lab 4 Organisms 2. Safranin couterstain of endospore staining procedure; 6. Endospore staining is a differential stain that aims at detecting, identifying and differentiating an endospore from the vegetative cell (an underdeveloped endospore). 1. The Bacillus cereus organism appeared as red rod-shaped chains of cells, measuring 5 um long and 1 um wide. Recognize endospores as the "structures" observed in an endospore stain preparation. Most failed endospore stains occur because the stain was allowed to completely evaporate during the procedure. Endospore Stain Results. Purpose: To differentiate between organisms that can produce endospores and those that cannot. There has been some concern about using heat in a teaching environment for safety reasons. Spores were present in the cells, with a short rod-like shape and green color. State the function of bacterial endospores. 2. This indicates that cells possess the ability to produce spores. Steam from heated water bath supplies heat to drive green stain into endospores; 4 & 5. In the endospore stain experiment (Table 2), a slide of Bacillus cereus was observed under 1000X magnification using oil immersion. Stain with malachite green solution for 10 minutes; Rinse thoroughly with water and dry briefly; Counter stain with safranin solution for 15 seconds; Rinse thoroughly with water and blot dry; Look at cells under 40X; Results. 1. 1, 2 & 3. RESULTS. Lab 7: Endospore Stain and Bacterial Motility Last updated; Save as PDF Page ID 3446; Contributors and Attributions; A. ENDOSPORE STAIN . However, positive results of endospore stain specify that the organism is a type of spore-forming or endospore encompassing organism. Identify a bacterium as an endospore-containing Clostridium by its "tennis racquet" appearance. ... Gram stain of Serratia marcescens. RESULTS . 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endospore stain results

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